The basic components of Loudi plant cell laboratory construction

What should be paid attention to in the construction of Loudi Plant Cell Laboratory?SICOLAB chats with you about the construction knowledge

I.Plant Cell LabConstruction site

The ideal tissue culture laboratory should be established in a quiet, clean place away from pollution sources, preferably in the upwind direction of the prevailing wind all the year round, and minimize thePollution.The tissue culture laboratory for large-scale production is best located in a place with convenient transportation to facilitate the transportation of cultured products.

Second,Plant finestrong>CellLaboratory construction requires two aspects:

One ​​is the nature of the experiment, whether it is productive or research, basic or higher

The second is the scale of the laboratory, which mainly depends on the funding and the nature of the experiment.

Three,Plant Cell LabConstruction Workflow

The cell culture laboratory should be able to perform six tasks: aseptic operation, incubation, preparation, cleaning, disinfection and sterilization, storage

Four.Plant Cell LabBuilding Composition

1. Washroom

According to the workload is too small to determine the size of the base, there is a dedicated washing sink on one side of the laboratory for cleaning glassware.The central laboratory bench should also be equipped with a sink for cleaning small glassware.If the workload is large, you can purchase a bottle washer.Prepare a lotion tank, which is specially used for washing glassware that requires high cleanliness.In addition, water racks, drying boxes, cabinets, ultrasonic cleaners, etc. should also be configured.The ground should be moisture resistant and well drained.

2.Medium preparation room

Main instruments and equipment are: refrigerator, balance, microwave oven, pH meter, medium dispenser, medicine cabinet, instrument cabinet, air pump, electric furnace, various specifications of culture flasks, petri dishes, pipettes, beakers, measuring cylinders, volumetric flasks, storage bottles, etc.

3.Sanitizing Roomstrong>

Equipped with laboratory bench, autoclave, exhaust and fire extinguishing equipment, bacterial filtration equipment, dry heat disinfection cabinet, electric furnace, etc.The selection of sterilizers should be based on different requirements to choose different types of sterilizers.A small medical portable autoclave can be used in general laboratories, and a vertical automatic pressure and temperature sterilizer can be used in larger laboratories.A large horizontal sterilizer can be used in productive laboratories.If there are no conditions, the above workshops can be combined into one preparation room. The requirements are that the installation and arrangement of equipment should be reasonable, the room should be spacious, bright and ventilated, and the floor should be easy to clean and non-slip.

4.Sterile Operating Room

The sterile operating room is mainly used for the inoculation of experimental materials, so it is also called the inoculation room.Usually consists of two rooms, the outer room is a buffer room, which is used for preparation and work, as well as to prevent pollution.The door of the buffer room should be staggered from the door of the inoculation room, and the two doors should not be opened at the same time to ensure that the sterile room does not bring in bacteria due to the door opening and the entry and exit of people.The buffer room should be equipped with a sink, a laboratory table, a shoe and hat rack, a cabinet, and a UV lamp.The inner wall of the sterile operating room should be decorated with plastic steel plates or ceramic tiles, and the staff should put on sterilized work clothes and slippers before entering the operating room.The room should be equipped with ultra-clean workbench, ultraviolet lamp, dissection mirror, various inoculation equipment, etc.

5.Incubator.

The culture room is the place where the inoculated material is cultured and grown.The size of the culture chamber can be determined according to the size, number and other auxiliary equipment of the culture rack.Its design is based on the principle of making full use of space and saving energy.The height is slightly higher than the culture rack, and the surrounding walls are required to have thermal insulation and fire resistance.

V.Plant Cell LabBuild Configuration Requirements

Cultivation racks, incubators, shakers, culture containers (Petri dishes, Erlenmeyer flasks, test tubes, culture flasks. Glass containers), metal utensils (tweezers, scissors, scalpel, inoculation spatula), plastic utensils (various parafilms, lids, plastic trays and other experimental utensils).